Preliminary Effects Of Olive Mill Wastewater On The Glucose ‎absorption In Mice ‎

Olive mill wastewater (OMWW) is the liquid by-product ‎generated during olive oil production. The aim of this study is to ‎investigate the effect of OMWW on intestinal glucose absorption ‎in mice, notably for treating diabetes. Oral glucose tolerance test ‎‎(OGTT) is a medical test in which glucose is given and blood ‎samples taken afterward to determine how quickly it is cleared ‎from the blood. This test was performed in Male C57BL/6J mice ‎weighing 20–25 g. Gavages of mice with glucose were ‎performed after a 16-h fast using a D-glucose solution (1 g/Kg) ‎without or with OMWW, in dose range of 0.5– 4 g/kg of body ‎weight. The bleeds were further taken at 15, 30, 60, and 120 min ‎after oral glucose administration. These experiments were ‎performed at least with 6 individual animals. Oral glucose ‎tolerance test carried out in mice showed that oral administration ‎of OMWW decreased glycemia at all tested concentration, which ‎is significant at concentration of 4g/Kg of body weight. These ‎results suggest OMWW have potentially hypoglycemic effect in ‎mice.‎