Kinetic Study Of Bio-demineralization And Bio-‎deproteinization Of Shrimp Biowaste For Chitin Recovery

Demineralization (DM) and deproteinization (DP) of shrimp shell ‎Parapenaeus longirostris using Lactobacillus helveticus, depends ‎on the composition of culture medium, and temperature of ‎incubation. In a synthetic medium containing glucose, completely ‎different conditions are required for chitin recovery: 300 g/L of ‎glucose and 35°C for maximal DM (60%) and 80 g/L of glucose ‎and 30°C for a maximum of DP (70%). The use of date’s juice ‎shows that it is possible to extract chitin, in a single step, unlike ‎the simple medium. The richness of the dates juice in mineral ‎elements and the high concentration of reducing sugars (200 ‎g/L) allowed for a maximum activity of proteolytic enzymes ‎favored by a pH maintained constant at around pH 6 for 29h of ‎fermentation, and a significant acidification expressed by a ‎minimum pH of 4.7 which are reflected by rates of 83% and 63% ‎of deproteinization and demineralization, respectively.‎